Research area

Stem Cells & Regenerative Medicine

Mesenchymal stem cells, preconditioning, secretome, lysates and exosomes for tissue repair.

This painting depicts the VegF signaling pathway and some of its consequences. Blood serum is at upper left, with VegF in red — David S. Goodsell, RCSB PDB · CC BY 4.0

Overview

Mesenchymal stem/stromal cells (MSCs) from bone marrow, adipose tissue, Wharton’s jelly and placenta are widely studied for their paracrine effects. Much current research focuses less on the cells themselves and more on what they release — conditioned medium, secretome, lysates and extracellular vesicles.

Good MSC research depends on careful characterisation, consistent culture conditions and controls that separate the effect of the product from the effect of the culture medium.

Questions we can help you answer

Which MSC source and passage range suit my question?
How do I show that an effect comes from the secretome and not the medium?
Which characterisation markers and assays will reviewers expect?
How should exosome isolation be validated and reported?

Methods we advise on

MSC isolation and culture
Preconditioning designs
Conditioned-medium and lysate preparation
Exosome isolation
Differentiation assays
Apoptosis, angiogenesis and oxidative-stress readouts

Please note

Regenerative-medicine research is pre-clinical unless conducted in an approved clinical trial. Nothing on this site is advice about stem-cell treatments for patients.
Work with human tissue or animals requires the relevant institutional ethics approvals.

Relevant services

This painting includes three types of filaments that make up the cytoskeleton: a microtubule (the largest), an intermediate filament (the knobby one), and two actin filaments (the smallest ones). The large blue molecules are ribosomes , busy in their task of synthesizing proteins — Goodsell · RCSB PDB · CC BY

Scientific & Research Consulting

Sharpen the research question, choose a defensible design and plan the work before time and reagents are spent.

For:: Postgraduate researchers and early-career scientists
Lead:: Scientific Lead

Explore service

This painting shows a cross-section through an Escherichia coli cell. The characteristic two-membrane cell wall of gram-negative bacteria is shown in green, with many lipopolysaccharide chains extending from the surface and a network of cross-linked peptidoglycan strands between the membranes — Goodsell · RCSB PDB · CC BY

Wet-Lab Strategy & Experimental Research Support

Experimental planning, assay design, troubleshooting and interpretation from a scientist with long bench experience. Hands-on work is coordinated only with qualified external laboratories.

For:: Postgraduate students designing or troubleshooting experiments
Lead:: Scientific Lead

Explore service

This painting is part of “VAX,” a series of paintings exploring the molecular basis of vaccines. These paintings are designed to be accurate representations of the biological processes, but they also serve as a personal celebration of a miracle of modern medicine — Goodsell · RCSB PDB · CC BY

Thesis, Dissertation & Research Project Support

Ethical, methodology-first support for BS, MSc, MPhil and PhD researchers. You remain the author; we help you plan, analyse, present and defend your own work.

For:: BS and MSc students starting a research project
Lead:: Scientific Lead

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Tell us what you’re working on.

Send a short, confidential summary. We reply with an initial scope assessment, and you agree deliverables, timeline and price before anything starts.

Discuss your research